A test of the model to predict unusually stable RNA hairpin loop stability
نویسندگان
چکیده
منابع مشابه
A test of the model to predict unusually stable RNA hairpin loop stability.
To investigate the accuracy of a model [Giese et al., 1998, Biochemistry37:1094-1100 and Mathews et al., 1999, JMol Biol 288:911-940] that predicts the stability of RNA hairpin loops, optical melting studies were conducted on sets of hairpins previously determined to have unusually stable thermodynamic parameters. Included were the tetraloops GNRA and UNCG (where N is any nucleotide and R is a ...
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Fifteen RNA hairpins that share the same stem sequence and have homopolymer loops of A, C and U residues which vary in length from three to nine nucleotides were synthesized and their thermal stabilities determined. Tm varies as a function of loop size but is almost independent of loop composition. Loops of four or five nucleotides are found to be the most stable loop size. This is consistent w...
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The solution structure of a d[C(TTCG)G] DNA hairpin and comparison to the unusually stable RNA analogue.
The solution structure of the DNA analogue of the unusually stable r[C(UUCG)G] RNA hairpin, 5'-d[GGA-C(TTCG)GTCC]-3', has been determined by NMR spectroscopy, and its structure has been compared to that of the RNA molecule. The RNA molecule is compact and rigid with a highly structured loop. However, the DNA molecule is much less structured. The DNA hairpin contains a B-form stem of four base p...
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ژورنال
عنوان ژورنال: RNA
سال: 2000
ISSN: 1355-8382
DOI: 10.1017/s1355838200992495